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1Department of Pharmacology and Systems Physiology, College of Medicine,University of Cincinnati, 2Division of Endocrinology,Cincinnati Children’s Hospital Medical Center, 3Division of Developmental Biology,Cincinnati Children’s Hospital Medical Center, 4Department of Pediatrics, College of Medicine,University of Cincinnati
Chapters
- 00:04Title
- 00:47Liver Perfusion and Extraction
- 02:45Primary Mouse Hepatocyte Isolation
- 04:07Polyacrylamide Gel Electrophoresis (PAGE) Analysis
- 06:06Results: Representative Nascent Protein Expression Analyses
- 07:31Conclusion
Her presenterer vi en protokoll for isolasjon hepatocytes sunn og funksjonelle hovedknappen på musen. Instruksjoner for å oppdage hepatic begynnende proteinsyntese av ikke-radioaktivt merking underlaget ble gitt til å forstå mekanismene bak proteinsyntese i sammenheng med energi-metabolisme homeostase i leveren.
