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En rotte Methyl-Seq plattform å identifisere Epigenetic endringer knyttet til Stress eksponering
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal Biochemistry
A Rat Methyl-Seq Platform to Identify Epigenetic Changes Associated with Stress Exposure
DOI:

09:06 min

October 24, 2018

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Chapters

  • 00:04Title
  • 01:04Sample Preparation
  • 01:54Hybridization
  • 04:16Bisulfite Conversion
  • 05:29Indexing
  • 06:06Validation by Bisulfite Pyrosequencing
  • 07:18Results: Stress-associated DNA Methylation Changes are verified by Bisulfite Pyrosequencing
  • 08:03Conclusion

Summary

Automatic Translation

Her beskriver vi protokollen og implementering av Methyl-Seq, en epigenomic plattform, med en rotte modell for å identifisere epigenetic endringer forbundet med kronisk stress eksponering. Resultatene viser at rotte Methyl-Seq plattformen er stand til å oppdage metylering forskjellene som framkommer stress eksponering i rotter.

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