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Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
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Chapters
- 00:05Title
- 00:55RT-LAMP for Detection of Zika-infected Mosquitoes Using Q-paper Technology
- 02:23Testing Lyophilized RT-LAMP Reagents on Urine Samples Containing Zika
- 04:05Results: Singleplex and Multiplex RT-LAMP for Detection of Zika, Chikungunya, and Dengue 1
- 05:40Conclusion
Current multiplexed diagnostics to detect Zika, chikungunya, and dengue viruses require complex sample preparation and expensive instrumentation, and are difficult to use in low resource environments. We show a diagnostic that uses isothermal amplification with target-specific strand displaceable probes to detect and differentiate these viruses with high sensitivity and specificity.
