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Humanperifert blod nøytrofil isolasjon for å forhøre Parkinsons tilknyttede LRRK2 Kinase Pathway ved å vurdere Rab10 fosforylering
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1MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences,University of Dundee, 2The Michael J. Fox Foundation for Parkinson’s Research, 3Department of Medical and Molecular Genetics,Indiana University School of Medicine, 4Morton and Gloria Shulman Movement Disorders Centre and the Edmond J. Safra Program in Parkinson’s Disease, Toronto Western Hospital,University of Toronto, 5Division of Cell Signalling and Immunology, School of Life Sciences,University of Dundee, 6Division of Molecular and Clinical Medicine, School of Medicine, Ninewells Hospital and Medical School,University of Dundee
Chapters
- 00:01Introduction
- 00:48Neutrophil Isolation from Human Peripheral Blood
- 07:18LRRK2 Kinase Inhibitor Treatment
- 10:33Results
- 12:14Conclusion
Mutasjoner i leucine rike gjenta kinase 2 genet (LRRK2) forårsake arvelig Parkinsons sykdom. Vi har utviklet en enkel og robust metode for å vurdere LRRK2-kontrollert fosforylering av Rab10 i humane perifere blodnøytrofiler. Dette kan bidra til å identifisere personer med økt LRRK2 kinase pathway aktivitet.
