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Journal
/
Developmental Biology
/
达尼奥
胚胎发生过程中的上眼硫可视化
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Visualization of the Superior Ocular Sulcus during
Danio rerio
Embryogenesis
Please note that all translations are automatically generated.
Click here for the English version.
达尼奥
胚胎发生过程中的上眼硫可视化
DOI:
10.3791/59259-v
•
08:03 min
•
March 27, 2019
•
Kevin H. Yoon
,
Sonya A. Widen
2
,
Melissa M. Wilson
,
Jennifer C. Hocking
3,4,5
,
Andrew J. Waskiewicz
2,6
1
Department of Biological Sciences
,
University of Alberta
,
2
Women & Children’s Health Research Institute
,
University of Alberta
,
3
Division of Anatomy, Department of Surgery
,
University of Alberta
,
4
Department of Cell Biology
,
University of Alberta
,
5
Department of Medical Genetics
,
University of Alberta
,
6
Neuroscience and Mental Health Research Institute
,
University of Alberta
Chapters
00:04
Title
00:28
Superior Ocular Sulcus (SOS) Visualization
03:15
Whole-Mount Immunofluorescent Laminin Staining
06:04
Results: Representative Zebrafish SOS Imaging
07:31
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们提出了一系列标准化的协议, 以观察上眼沟, 最近确定的, 进化保存的结构在脊椎动物的眼睛。利用斑马鱼幼虫, 我们展示了必要的技术, 以确定有助于形成和关闭的上眼沟的因素。
Tags
Zebrafish
Embryogenesis
Superior Ocular Sulcus
SOS
Visualization
Dissecting Microscope
Compound Microscope
Confocal Microscope
1-Phenyl-2-thiourea
Agarose
E3 Medium
Embryo Positioning
MRNA Injection
Enhanced GFP
Fluorescent Microscopy
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