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JoVE Journal Developmental Biology
Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence
 

Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence

Article DOI: 10.3791/59413-v • 06:27 min • April 17th, 2019
April 17th, 2019 •
1,2,3, 1,2,3
1Department of Medicine, Division of Cardiovascular Medicine, Vanderbilt University Medical Center, 2Department of Cell and Developmental Biology, Vanderbilt University, 3Vanderbilt Center for Stem Cell Biology, Vanderbilt University

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Summary
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We present the protocols to examine mouse heart development using whole mount epifluorescent microscopy on mouse embryos dissected from ventricular specific MLC-2v-tdTomato reporter knock-in mice. This method allows us to directly visualize each stage of the ventricular formation during mouse heart development without labor-intensive histochemical methods.

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Keywords: Cardiac Chamber Development Mouse Embryogenesis Whole Mount Epifluorescence MLC-2v-tdTomato Heart Tube Formation Looping Chamber Formation Fluorescent Reporter Dissection Genotyping Epifluorescent Imaging
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