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Analysis of Cell Differentiation, Morphogenesis, and Patterning During Chicken Embryogenesis Using the Soaked-Bead Assay
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Chapters
- 00:04Introduction
- 00:48Egg Incubation and Embryo Staging
- 02:08Bead Preparation
- 04:34Embryo Manipulation and Interdigit Implantation
- 05:42Results: Evaluation of Cell Behavior in the Embryonic Chick Limb Using Soaked Beads
- 06:20Conclusion
The soaked bead assay involves targeted delivery of test reagent at any developmental time point to study the regulation of cell differentiation and morphogenesis. A detailed protocol, applicable to any experimental animal model, for preparing three different types of soaked beads and implanting these in the interdigit of a chicken embryo is presented.
