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将鼠皮皮和皮米与热解辛分离，以检测部位特异性炎症mRNA和蛋白质
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Immunology and Infection
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JoVE Journal Immunology and Infection
Separation of Rat Epidermis and Dermis with Thermolysin to Detect Site-Specific Inflammatory mRNA and Protein
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将鼠皮皮和皮米与热解辛分离，以检测部位特异性炎症mRNA和蛋白质

DOI:
10.3791/59708-v

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08:45 min

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September 29, 2021

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, , , , ,
1Department of Anatomy and Cell Biology,Oklahoma State University Center for Health Sciences, 2Institute for Shock Physics,Washington State University

Chapters

  • 00:04Introduction
  • 01:21Carrageenan-Induced Inflammation (C-II)
  • 02:31Thermolysin Epidermis and Dermis Separation
  • 04:11Tinctorial Histology
  • 05:43Results: Representative Site-Specific Inflammatory mRNA and Protein Detection
  • 07:40Conclusion

Summary

Automatic Translation

Automatic Translation

这里介绍的是一个表皮与真皮分离的协议，用于评估炎症调解器的产生。炎症后，大鼠后爪表皮在4°C下由热利辛从真皮中分离出来。 然后，通过RT-PCR进行表皮分析，通过西方污点和免疫造血术进行蛋白质评估。

Tags

EpidermisDermisThermolysinSkin InflammationInflammatory MediatorsNeurotrophinsWound HealingSkin DiseaseChronic WoundsBurnsEpithelial SurfacesGI TractCorneaSprague Dawley RatLambda CarrageenanEdemaGlabrous Hind PawMicrodissection ForcepsDMEMStratum Corneum

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