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Biology
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使用光可转换的登德拉
2,在老化的
蛋白质周转量中,在体内C.Elegans
JoVE Journal
Biology
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JoVE Journal
Biology
In Vivo Quantification of Protein Turnover in Aging
C. Elegans
using Photoconvertible Dendra2
Please note that all translations are automatically generated.
Click here for the English version.
使用光可转换的登德拉
2,在老化的
蛋白质周转量中,在体内C.Elegans
DOI:
10.3791/61196-v
•
09:45 min
•
June 13, 2020
•
Maria Lucia Pigazzini
2
,
Janine Kirstein
3
1
Leibniz Research Institute for Molecular Pharmacology im Forschungsverbund
,
2
NeuroCure Cluster of Excellence
,
Charité – Universitätsmedizin Berlin
,
3
Department of Biology and Chemistry
,
University of Bremen
Chapters
00:04
Introduction
00:50
Microscope Slide Preparation
01:38
Confocal Microscope Setup
03:17
C. elegans
Mounting
04:06
Green Dendra2 Conversion: Data Acquisition at Time Zero
05:35
Red Dendra2 Conversion: Data Acquisition at Second Time Point
06:13
Converted Dendra2 Image Analysis
07:17
Results: Representative Huntingtin (HTT)-D2 Conversion and Degradation
09:08
Conclusion
Summary
Automatic Translation
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Automatic Translation
这里介绍的是一个协议,用于监测蛋白质亨廷顿蛋白的降解,该蛋白与光可转换的荧光素Dendra2有关。
Tags
Protein Turnover
Dendra2
C. Elegans
Photoconversion
In Vivo
Quantification
Aging
Proteostasis
Trafficking
Microscopy
Agarose Pad
Confocal
Laser
Acquisition Settings
Conversion
Bleaching
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