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使用光可转换的登德拉2，在老化的蛋白质周转量中，在体内C.Elegans

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JoVE Journal Biology

In Vivo Quantification of Protein Turnover in Aging C. Elegans using Photoconvertible Dendra2

Please note that all translations are automatically generated. Click here for the English version.

使用光可转换的登德拉2，在老化的蛋白质周转量中，在体内C.Elegans

DOI:

10.3791/61196-v

•

09:45 min

•

June 13, 2020

•

Maria Lucia Pigazzini², Janine Kirstein³

¹Leibniz Research Institute for Molecular Pharmacology im Forschungsverbund, ²NeuroCure Cluster of Excellence,Charité – Universitätsmedizin Berlin, ³Department of Biology and Chemistry,University of Bremen

Chapters

00:04Introduction
00:50Microscope Slide Preparation
01:38Confocal Microscope Setup
03:17C. elegans Mounting
04:06Green Dendra2 Conversion: Data Acquisition at Time Zero
05:35Red Dendra2 Conversion: Data Acquisition at Second Time Point
06:13Converted Dendra2 Image Analysis
07:17Results: Representative Huntingtin (HTT)-D2 Conversion and Degradation
09:08Conclusion

Summary

Automatic Translation

这里介绍的是一个协议，用于监测蛋白质亨廷顿蛋白的降解，该蛋白与光可转换的荧光素Dendra2有关。

Tags

Keywords: Protein Turnover Dendra2 C. Elegans Photoconversion In Vivo Quantification Aging Proteostasis Trafficking Microscopy Agarose Pad Confocal Laser Acquisition Settings Conversion Bleaching

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