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Evaluering av celledød ved hjelp av cellefri supernatant av probiotika i tredimensjonale sfæroidkulturer av kolorektale kreftceller
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1Microbiological Analysis Team, Group for Biometrology,Korea Research Institute of Standards and Science (KRISS), 2College of Pharmacy,Chungnam National University (CNU), 3Stem Cell Research Center,Korea Research Institute of Bioscience and Biotechnology (KRIBB), 4Convergent Research Center for Emerging Virus Infection,Korea Research Institute of Chemical Technology (KRICT), 5Department of Bio-Analysis Science,University of Science & Technology (UST)
Chapters
- 00:04Introduction
- 00:34Bacterial Cell Culture and Lactobacillus Cell-Free Supernatant (LCFS) Preparation
- 02:18Colorectal Cancer Cell Line Preparation
- 03:12Spheroid Formation
- 03:493D colorectal Cancer Cell LCFS Treatment
- 04:26Results: Representative Effects of LCFS Co-Culture on Spheroid Formation and Viability
- 05:22Conclusion
Her presenteres metoder for å forstå antikrefteffekter av Lactobacillus cellefri supernatant (LCFS). Kolorektal kreftcellelinjer viser celledødsfall når de behandles med LCFS i 3D-kulturer. Prosessen med å generere sfæroider kan optimaliseres avhengig av stillaset, og analysemetodene som presenteres er nyttige for å evaluere de involverte signalveiene.
Tags
Cell DeathCell-free SupernatantProbioticsThree-dimensional SpheroidColorectal CancerLactobacillusAnti-cancer EffectIn Vivo MimicryMRS AgarAnaerobic ChamberMRS BrothL-cysteinePBSShaking IncubatorOD620CentrifugationRPMI 1640Fetal Bovine Serum0.22 Micron StrainerColorectal Cancer Cell LinesMonolayersTrypsin-EDTAGrowth Medium
