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Fully Human Tumor-based Matrix in Three-dimensional Spheroid Invasion Assay
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Cancer Research
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JoVE Journal Cancer Research
Fully Human Tumor-based Matrix in Three-dimensional Spheroid Invasion Assay

Fully Human Tumor-based Matrix in Three-dimensional Spheroid Invasion Assay

DOI:
10.3791/59567-v

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08:15 min

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May 07, 2019

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, , , , , ,
1Department of Oral and Maxillofacial Diseases, Clinicum, Faculty of Medicine,University of Helsinki, 2Cancer and Translational Medicine Research Unit,University of Oulu, 3Medical Research Center,Oulu University Horspital, 4Helsinki University Hospital

Chapters

  • 00:04Title
  • 01:10Multicellular Tumor Spheroid Generation and 3D Spheroid Invasion Assay
  • 02:53Image Segmentation With Ilastik
  • 04:39Area Analysis with Fiji ImageJ
  • 06:23Results: Representative Cell Movement in a 3D Invasion Model
  • 07:36Conclusion

Summary

Automatic Translation

Automatic Translation

Tumor microenvironment is an essential part of cancer growth and invasion. To mimic carcinoma progression, a biologically relevant human matrix is needed. This protocol introduces an improvement for the in vitro three-dimensional spheroid invasion assay by applying a human leiomyoma-based matrix. The protocol also introduces a computer-based cell invasion analysis.

Tags

3D Spheroid Invasion AssayTumor MicroenvironmentHuman Tumor MatrixPatient-derived Solid Cancer SamplesHigh-throughput Drug TestingLive-cell AnalysisWound HealingTransfer AssaysFibrinogenThrombinAprotininMulticellular Tumor Spheroid GenerationMyoma-derived Matrix

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