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Isolamento de Fluidos Proximais para Investigar o Microambiente Tumoral do Adenocarcinoma Pancreático
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1Section of Pancreatic Surgery,Humanitas Clinical and Research Center-IRCCS, 2Department of Immunology and Inflammation,Humanitas Clinical and Research Center-IRCCS, 3Department of Biomedical Sciences,Humanitas University, 4Giotto Biotech S.R.L., 5Section of Bioanalytical Chemistry, Division of Systems Medicine, Department of Metabolism, Digestion and Reproduction,Imperial College London, 6National Phenome Centre, Department of Metabolism, Digestion and Reproduction, Faculty of Medicine,Imperial College London, 7Department of Medical Biotechnology and Translational Medicine,University of Milan
Chapters
- 00:04Introduction
- 01:03Isolation and Processing of Pancreatic Juice
- 02:20Isolation of Tumor Interstitial Fluid (TIF) from Subcutaneous Tumors
- 04:03Results: Metabolomic Content in Pancreatic Juice
- 05:14Conclusion
O suco pancreático é uma fonte preciosa de biomarcadores para o câncer de pâncreas humano. Descrevemos aqui um método para o procedimento de coleta intraoperatória. Para superar o desafio de adotar esse procedimento em modelos murinos, sugerimos uma amostra alternativa, o líquido intersticial tumoral, e descrevemos aqui dois protocolos para seu isolamento.
