Journal
/
Immunology and Infection
/
Systematic Scoring Analysis for Intestinal Inflammation in a Murine Dextran Sodium Sulfate-Induced Colitis Model
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Systematic Scoring Analysis for Intestinal Inflammation in a Murine Dextran Sodium Sulfate-Induced Colitis Model

Systematic Scoring Analysis for Intestinal Inflammation in a Murine Dextran Sodium Sulfate-Induced Colitis Model

DOI:
10.3791/62135-v

•

09:11 min

•

February 14, 2021

•

, , , , ,
1Department of Pathology,University of Michigan, 2Department of Surgery, Klinikum Rechts der Isar,Technische Universität München, 3Wallenberg Centre for Molecular Medicine,Linköping University, 4Department of Biomedical and Clinical Sciences (BKV),Linköping University

Chapters

  • 00:04Introduction
  • 00:50Tissue Harvest
  • 02:24Preparation of Swiss Rolls
  • 03:57Digital Scanning and Analysis
  • 06:33Results: Histological Colitis Score and Percentage of Injury and Ulceration Correlates with Body Weight and Stool Index During DSS Experimental Colitis
  • 08:30Conclusion

Summary

Automatic Translation

Automatic Translation

Systematic scoring of intestinal inflammation using a free computer-assisted system is a powerful tool to quantitatively compare histopathological changes in colitis models characterized by the presence of ulcers and inflammatory changes. Histological colitis score evaluation strengthens clinical observations and facilitates data interpretation.

Tags

Keyword Extraction:Systematic Scoring AnalysisIntestinal InflammationMurine Dextran Sodium Sulfate-induced Colitis ModelMucosal InjuryHistological InterpretationQuantitative AnalysisIntestinesMiceColitisEuthanized MouseDissecting PadAbdominal SkinPeritoneumLarge IntestinePelvic BoneColonPBSWestern BlotPCRImmunofluorescence MicroscopyNeutral Buffered Formalin Solution

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/62135?article_type=v

Trichuris muris Infection: A Model of Type 2 Immunity and Inflammation in the Gut
check_url/62135?article_type=v

Flow Cytometry Analysis of Immune Cells Within Murine Aortas
check_url/62135?article_type=v

Analysis of Pulmonary Dendritic Cell Maturation and Migration during Allergic Airway Inflammation
check_url/62135?article_type=v

The Citrobacter rodentium Mouse Model: Studying Pathogen and Host Contributions to Infectious Colitis
check_url/62135?article_type=v

A Mouse Model for Pathogen-induced Chronic Inflammation at Local and Systemic Sites
check_url/62135?article_type=v

Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice
check_url/62135?article_type=v

An Optimized Method for Isolating and Expanding Invariant Natural Killer T Cells from Mouse Spleen
check_url/62135?article_type=v

Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells
check_url/62135?article_type=v

Induction and Scoring of Graft-Versus-Host Disease in a Xenogeneic Murine Model and Quantification of Human T Cells in Mouse Tissues using Digital PCR
check_url/62135?article_type=v

Intravital Imaging of Intraepithelial Lymphocytes in Murine Small Intestine

Read Article