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使用扫描电子显微镜可视化膜褶皱形成
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JoVE Journal Medicine
Visualizing Membrane Ruffle Formation using Scanning Electron Microscopy
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使用扫描电子显微镜可视化膜褶皱形成

DOI:
10.3791/62658-v

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08:05 min

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May 27, 2021

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, , ,
1Vascular Biology Center,Medical College of Georgia at Augusta University, 2Department of Cellular Biology and Anatomy,Medical College of Georgia at Augusta University, 3Department of Pharmacology and Toxicology,Medical College of Georgia at Augusta University

Chapters

  • 00:04Introduction
  • 00:47Cell Treatment
  • 01:56Scanning Electron Microscopy Fixation
  • 02:48Critical Point Drying
  • 05:16Imaging and Quantification
  • 05:58Results: Visualization and Quantification of Membrane Ruffle Formation
  • 07:34Conclusion

Summary

Automatic Translation

Automatic Translation

大旋毛细胞增多症是一种高度保守的内吞作用过程，由形成富含F的片状膜突起（也称为膜褶皱）而引发。大旋细胞溶质内化速率增加与各种病理疾病有关。该协议提出了一种使用扫描电子显微镜 在体外 量化膜褶皱形成的方法。

Tags

Membrane RufflesMacropinocytosisScanning Electron MicroscopyCell SurfaceSEM ImagingMembrane ActivitiesMacropinocytic CupsMacrophagesPMAM-CSFCritical Point Drying

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