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Visualizing Membrane Ruffle Formation using Scanning Electron Microscopy
 

Visualizing Membrane Ruffle Formation using Scanning Electron Microscopy

Article DOI: 10.3791/62658-v • 08:05 min • May 27th, 2021
May 27th, 2021 •
1, 1, 2, 1,3
1Vascular Biology Center, Medical College of Georgia at Augusta University, 2Department of Cellular Biology and Anatomy, Medical College of Georgia at Augusta University, 3Department of Pharmacology and Toxicology, Medical College of Georgia at Augusta University

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Summary
Automatic Translation

Macropinocytosis is a highly conserved endocytic process initiated by the formation of F-actin-rich sheet-like membrane projections, also known as membrane ruffles. Increased rate of macropinocytotic solute internalization has been implicated in various pathological conditions. This protocol presents a method to quantify membrane ruffle formation in vitro using scanning electron microscopy.

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Keywords: Membrane Ruffles Macropinocytosis Scanning Electron Microscopy Cell Surface SEM Imaging Membrane Activities Macropinocytic Cups Macrophages PMA M-CSF Critical Point Drying
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