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用立方体进行全肾三维染色
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Medicine
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JoVE Journal Medicine
Whole-Kidney Three-Dimensional Staining with CUBIC
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用立方体进行全肾三维染色

DOI:
10.3791/63986-v

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04:31 min

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July 18, 2022

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,
1Division of Nephrology and Endocrinology,The University of Tokyo

Chapters

  • 00:05Introduction
  • 00:37Kidney Fixation, Decolorization, and De-Lipidation
  • 01:45Whole-Mount Immunofluorescent Staining and Refractive Index (RI) Matching
  • 03:03Results: Whole-Mount Immunofluorescent Staining for Three-Dimensional (3D) Kidney Imaging
  • 04:00Conclusion

Summary

Automatic Translation

Automatic Translation

本协议描述了用于三维（3D）肾脏成像的组织清除方法和全安装免疫荧光染色。这种技术可以为肾脏病理学提供宏观视角，从而带来新的生物学发现。

Tags

3D Kidney ImagingWhole-kidney StainingCUBICKidney ResearchOrgan StainingAntibody PenetrationDecolorizationDelipidationImmunostainingSympathetic NervesIschemia-reperfusion InjuryRefractive Index Matching

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