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Helnjure tredimensionell färgning med CUBIC
JoVE Journal
Medicine
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JoVE Journal Medicine
Whole-Kidney Three-Dimensional Staining with CUBIC
DOI:

04:31 min

July 18, 2022

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Chapters

  • 00:05Introduction
  • 00:37Kidney Fixation, Decolorization, and De-Lipidation
  • 01:45Whole-Mount Immunofluorescent Staining and Refractive Index (RI) Matching
  • 03:03Results: Whole-Mount Immunofluorescent Staining for Three-Dimensional (3D) Kidney Imaging
  • 04:00Conclusion

Summary

Automatic Translation

Detta protokoll beskriver en vävnadsrensningsmetod och helmonterad immunofluorescerande färgning för tredimensionell (3D) njuravbildning. Denna teknik kan erbjuda makroskopiska perspektiv inom njurpatologi, vilket leder till nya biologiska upptäckter.

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