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Cancer Research
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磷脂介质诱导的三维培养物转化
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Phospholipid Mediator Induced Transformation in Three-Dimensional Cultures
Please note that all translations are automatically generated.
Click here for the English version.
磷脂介质诱导的三维培养物转化
DOI:
10.3791/64146-v
•
08:02 min
•
July 27, 2022
•
Abhijith Kuttanamkuzhi
,
Libi Anandi
2
,
Vaishali Chakravarty
3
,
Mayurika Lahiri
1
Biology Department
,
Indian Institute of Science Education and Research
,
2
Department of Biology, Center for Genomics and Systems Biology
,
New York University
,
3
Enveda Therapeutics India Pvt Ltd.
Chapters
00:04
Introduction
00:59
Seeding MCF10A Cells in lrECM, PAF Treatment, and Re-Feeding with Fresh Media
03:00
Immunofluorescence Study to Detect Phenotypic Changes Induced by Prolonged PAF Exposure
06:05
Results: Nuclei of Cells in MCF10A Acini Stained with a Nuclear Stain
07:25
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本协议描述了未转化的乳腺上皮细胞系MCF10A的3D
“
顶部”培养物的建立,该培养物已被修改以研究血小板活化因子(PAF)诱导的转化。免疫荧光已被用于评估转化,并详细讨论。
Tags
3D Cell Culture
LrECM
Phospholipid Mediator
PAF
Breast Cancer
Biomarkers
Drug Targets
Tumorigenesis
Morphogenesis
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