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Biology

Published: July 21, 2023 doi: 10.3791/65393
Automatic Translation
*1, *2, 3, 2, 4, 5, 6, 1,2
1Department of Pharmaceutical Sciences, School of Pharmacy, Texas Tech University Health Sciences Center, 2Laboratory of Molecular Pharmacology, Department of Pharmacy, University of Patras, 3Division of Flow Cytometry and Division of MDx & Life Sciences, SB BioAnalytica, 4Basic Sciences Laboratory, Department of Physiotherapy, University of Peloponnese, 5Division of Hematology, Department of Internal Medicine, Medical School, University of Patras, 6School of Basic Pharmaceutical and Toxicological Sciences, College of Pharmacy, University of Louisiana at Monroe
* These authors contributed equally

Materials

Name Company Catalog Number Comments
0.2 μm Syringe Filters Fisher Scientific 09-719C
100 mm Tissue Culture Dishes Fisher Scientific FB012924
60 mm Tissue Culture Dishes Fisher Scientific FB012921
Animal Hair Clipper Wahl
Antibiotic-Antimycotic Solution 100x Fisher Scientific 15240-062
Blunt Forceps Fine Science Tools 11992-20
Bovine Serum Albumin Sigma-Aldrich A4919
Cell Strainer 40 μm Fisher Scientific 542040
Cell Strainer 70 μm Fisher Scientific 542070
CO2 Incubator
Collagen I, High Concentration Rat Tail Corning 354249 dilute in 0.02 M Acetic Acid in H2O
Collagenase Type II Life Technologies 17101-015
Dispase Life Technologies 17105-041
DMEM Fisher Scientific 11-995-073
DNAse I Solution (2,500 U/mL) Thermo Scientific 90083
Dynal MPC-L Magnetic Particle Concentrator Invitrogen 120-21D
EDTA Sigma-Aldrich 3690
Endothelial Cell Growth Base Medium & Supplement (LEC medium) R&D Systems CCM027
Euthanasia chamber Euthanex Corporation
Fine Forceps Fine Science Tools 11255-20
Fine Scissors-Sharp Fine Science Tools 14060-10
FITC anti-mouse F4/80 Antibody Biolegend 123107
Goat Anti-Rabbit IgG Magnetic Beads New England Biolabs S1432S
LARC-A E-Z Anesthesia Induction Chamber Euthanex Corporation
MagnaBind Goat Anti-Rabbit IgG Beads Thermo Scientific 21356
Paraformaldehyde Solution 4% in PBS Fisher Scientific AAJ19943K2
Phosphate Buffered Saline (PBS) Fisher Scientific SH30256FS
Rabbit Anti-Mouse LYVE-1 ReliaTech GmbH 103-PA50
Rotating/Shaking Incubator
Round-Bottom Polypropylene Tubes Corning 352063
Syringe Filters w 0.2 μm Pores Fisher Scientific 09-719C
Trypsin-EDTA Fisher Scientific 25-300-120

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References

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Cite this Article

Akwii, R. G., Lamprou, M.,… More

Akwii, R. G., Lamprou, M., Georgomanoli, M., Plevriti, A., Marazioti, A., Mouzaki, A., Mattheolabakis, G., Mikelis, C. M. Murine Dermal Lymphatic Endothelial Cell Isolation. J. Vis. Exp. (197), e65393, doi:10.3791/65393 (2023).

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