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JoVE Journal Bioengineering
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Bioengineering

Published: December 1, 2023 doi: 10.3791/66154
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1, 1, 1, 1
1Department of Biomedical Engineering, Institute for Complex Molecular Systems, Eindhoven University of Technology

Materials

Name Company Catalog Number Comments
1,1,3,3 tetramethyldisiloxane Merck Life Science NV MFCD00008256
4 channel digital input/output module WAGO Kontakttechnik GmbH 750-504
Acetone Boom Labs BOOMSKEUZW3
Analysis Software Eindhoven University of Technology https://github.com/SysBioOncology/BilayerMicrofluidicsAnalysis_JoVE
AZ 40XT 11D Merck Life Science NV  212299  Positive photoresist 
AZ 726 MIF developer Merck Life Science NV 10055824960 Developer for positive photoresist
Biopsy Punch, Rapid Core World Precision Instruments Germany, GMBH 504529 0.75 mm ID, W/Plunge
Blue food dye PME FC1036
Controller end module  WAGO Kontakttechnik GmbH  750-600
Ethernet Controller  WAGO Kontakttechnik GmbH  750-881
FC-40 Merck Millipore F9755-100ML
Fluigent flow unit Fluigent FLU-S-D
Fluigent pressure system  Fluigent  MFCS-EZ  0 - 2 bar
Fluorescein Merck Life Science NV MFCD00005050
Hot plate  Torrey Pines Scientific  HP61
Inverted microscope  Nikon Instruments  Eclipse Ti-E
Isopropanol Boom Labs BOOMSKEUZE3
LabVIEW (Software Version 20) Eindhoven University of Technology https://github.com/SysBioOncology/BilayerMicrofluidicsAnalysis_JoVE/tree/main/LabVIEW_8_inlet_device_
VERSION_1		 All files have been saved for LabVIEW version 20. It is advised to use this version or higher to open the files.
Luer stubs  Instech Laboratories, Inc.  LS23 23 ga, 0.5"
Male Luer to barb connectors  Cole Parmer  45505-32  3/32" ID
MasterFlex PTFE tubing Avator/VWR 48634
Microscope Slides VWR 470150-480
Microscope slides,  Plain Corning 2947-75X50
Mineral Oil Merck Millipore 330760-1L
mr DEV 600 Micro resist Technology R815100 Developer for negative photoresist
Oven Thermo Scientific  Heraeus T6P 50045757
Oxygen plasma asher Quorum Technologies K1050X
Photomask CAD/Art Services, Inc.
Photomask Design Eindhoven University of Technology (Adapted from Merten Lab, EPFL) https://github.com/SysBioOncology/BilayerMicrofluidicsAnalysis_JoVE/blob/main/8_inlet_JoVE_device_design.dwg
Pneumatic valve array FESTO 1x 8 valve array, Normally closed valves
Silicon Wafers Silicon Materials <1-0-0>, 100 mm diameter, 525 μm thickness
Single edge blades  GEM Scientific
Soft tubing Fluigent 1 mm ID, 3 mm OD
Spin coater  Laurell Technologies Corporation  WS-650MZ-23NPPB
Stereo microscope  Olympus Corporation  SZ61
SU-8 3050 Kayakli Advanced Materials Y311075 1000L1GL Negative photoresist
Sylgard 184 Silicone Elastomer Kit (PDMS) Dow 1317318
Syringe B Braun Injekt - F Fine Dosage Syringe 10303002
UV-LED exposure system Idonus UV-EXP150S-SYS
Vacuum pump  Vacuumbrand GmbH  MD1C
Weighing scales  Sartorius  M-prove

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Cite this Article

Yelleswarapu, M., Spinthaki, S., de… More

Yelleswarapu, M., Spinthaki, S., de Greef, T. F. A., Eduati, F. Bilayer Microfluidic Device for Combinatorial Plug Production. J. Vis. Exp. (202), e66154, doi:10.3791/66154 (2023).

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