Journal
/
Biology
/
处理火炬松PtGen2差异表达基因的cDNA微阵列
JoVE Journal
Biology
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Processing the Loblolly Pine PtGen2 cDNA Microarray
Please note that all translations are automatically generated. Click here for the English version.

处理火炬松PtGen2差异表达基因的cDNA微阵列

DOI:
10.3791/1182-v

•

07:01 min

•

March 20, 2009

•

, , ,
1Warnell School of Forestry and Natural Resources,University of Georgia (UGA), 2Instituto de Biologia Experimental e Tecnológica,Instituto Tecnologia Química e Biológica UNL, Av. da República

Chapters

  • 00:00Title
  • 00:43Microarray Slide Pre-Wash
  • 01:05Pre-Hybridization
  • 01:31Post Pre-Hybridization
  • 03:27Post-Hybridization Wash
  • 05:56Imaging
  • 06:39Conclusion

Summary

Automatic Translation

Automatic Translation

的cDNA微阵列PtGen2的基因表达研究开发火炬松，<em> P。火炬松</em>，和其他针叶树种。在这里，我们展示了前和杂交后处理和清洗技术，可以使用这个数组产生更好的一致性，减少了工件，和较低的背景。

Tags

Loblolly Pine PtGen2CDNA MicroarrayGene ExpressionConifer SpeciesGene DiscoveryNeedleStemCy-dye Labeled Conifer Target CDNAsAmplified And Non-amplified Indirect Labeling MethodsHybridizationWashing ConditionsHandling SlidesPre-hybridizationHybridization ProceduresTarget CDNA GenerationLabeling And Cleanup ProtocolsSoftware For Data ProcessingProprietary Print BufferSDS SolutionSalt RemovalLifterSlips™Labeled CDNA Loading

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/1182?article_type=v

Gibberella zeae子囊孢子生产和收集微阵列实验。
check_url/1182?article_type=v

微探针：操作和使用的局部表面处理
check_url/1182?article_type=v

从胚胎斑马鱼和cDNA合成的基因表达分析的RNA分离
check_url/1182?article_type=v

全球基因表达分析，使用斑马鱼的寡核苷酸芯片平台
check_url/1182?article_type=v

数字微流体进行自动蛋白质组学处理
check_url/1182?article_type=v

的RNA分离方法的改进火炬松（ P。火炬松 L）和其他针叶树种
check_url/1182?article_type=v

微阵列分析FOR酿酒酵母</em
check_url/1182?article_type=v

执行自定义的微RNA基因芯片实验
check_url/1182?article_type=v

前体mRNA底物的3'末端切割：用无细胞体系中的RNA加工反应分析<em>在体外</em
check_url/1182?article_type=v

同步加速器微CT高通量皮质骨样品采购和分析的剖面、科林和图像处理指南

Read Article