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Chapters
- 00:00Title
- 00:43Microarray Slide Pre-Wash
- 01:05Pre-Hybridization
- 01:31Post Pre-Hybridization
- 03:27Post-Hybridization Wash
- 05:56Imaging
- 06:39Conclusion
Den cDNA microarray PtGen2 har utvecklats för studier genuttryck i Loblolly tall,<em> P. taeda</em> Och andra barrträd arter. Här visar vi pre-och post-hybridisering hantering och tvätt tekniker som kan användas med denna array ge bättre konsistens, minskade artefakter, och lägre bakgrunder.
Tags
Loblolly Pine PtGen2CDNA MicroarrayGene ExpressionConifer SpeciesGene DiscoveryNeedleStemCy-dye Labeled Conifer Target CDNAsAmplified And Non-amplified Indirect Labeling MethodsHybridizationWashing ConditionsHandling SlidesPre-hybridizationHybridization ProceduresTarget CDNA GenerationLabeling And Cleanup ProtocolsSoftware For Data ProcessingProprietary Print BufferSDS SolutionSalt RemovalLifterSlips™Labeled CDNA Loading
