/

/

Measuring Replicative Life Span in the Budding Yeast

JoVE Journal
Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology

Measuring Replicative Life Span in the Budding Yeast

Measuring Replicative Life Span in the Budding Yeast

DOI:

•

12:41 min

•

June 25, 2009

•

Kristan K. Steffen, Brian K. Kennedy, Matt Kaeberlein

¹Department of Biochemistry,University of Washington, ²Department of Pathology,University of Washington

Chapters

00:05Title
00:26Introduction
00:49Prepare Strains and Plates for Replicative Life Span Analysis
03:35Position Cells for Replicative Life Span Analysis
06:22Obtain Virgin Daughter Cells for Life Span Analysis
08:37Measuring the Replicative Capacity of Individual Cells
11:18Representative Results
12:10Conclusion

Summary

Automatic Translation

In this article we present a general protocol for measuring the replicative life span of yeast mother cells.

Tags

Replicative Life Span Budding Yeast Aging Cellular Components Organelles Mortality Saccharomyces Cerevisiae Biology Of Aging Age-associated Phenotypes Life Span Paradigms Chronological Life Span Quiescence-like State Post-mitotic Cells Replicative Life Span Assay Daughter Cells Senescence Mitotically Active Cells Light Microscope

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Measuring Caenorhabditis elegans Life Span on Solid Media

Quantifying Yeast Chronological Life Span by Outgrowth of Aged Cells

Purification of the M. magneticum Strain AMB-1 Magnetosome Associated Protein MamAΔ41

Analysis of the Development of a Morphological Phenotype as a Function of Protein Concentration in Budding Yeast

Measuring Caenorhabditis elegans Life Span in 96 Well Microtiter Plates

Measuring the Kinetics of mRNA Transcription in Single Living Cells

Acquiring Fluorescence Time-lapse Movies of Budding Yeast and Analyzing Single-cell Dynamics using GRAFTS

Budding Yeast Protein Extraction and Purification for the Study of Function, Interactions, and Post-translational Modifications

Measuring the Osmotic Water Permeability Coefficient (P_f) of Spherical Cells: Isolated Plant Protoplasts as an Example

Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80

Read Article