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标准化的制备小鼠肺组织单细胞悬浮液使用gentleMACS Dissociator
JoVE Journal
Biology
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JoVE Journal Biology
Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator
DOI:

07:03 min

July 02, 2009

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Chapters

  • 00:04Title
  • 00:31Introduction
  • 01:01Preparing Enzyme Solutions and Buffers
  • 01:50Dissociating the Lung Tissue
  • 04:10Filtration
  • 05:35Representative Results
  • 06:07Conclusion

Summary

Automatic Translation

游离特定的组织类型的细胞,组织鼓动的具体参数,以获得一个可行的,可培养细胞的高容量。美天旎gentleMACS dissociator优化这个任务简单,实用的协议。在本出版物中使用本仪器对肺组织进行了解释。

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