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Microinjection Techniques for Studying Mitosis in the Drosophila melanogaster Syncytial Embryo

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Microinjection Techniques for Studying Mitosis in the Drosophila melanogaster Syncytial Embryo

Microinjection Techniques for Studying Mitosis in the Drosophila melanogaster Syncytial Embryo

DOI:

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09:25 min

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September 15, 2009

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Ingrid Brust-Mascher, Jonathan M. Scholey

¹Department of Molecular and Cellular Biology,University of California, Davis

Chapters

00:00Title
00:48Introduction
01:30Lay Cage and Needle Preparation
03:07Coverslip and Embryo Preparation
05:58Injecting and Imaging the Embryos
07:26Representative Embryo Injection Results
08:43Conclusion

Summary

Automatic Translation

This protocol describes the use of microinjection and high resolution imaging in the Drosophila melanogaster syncytial embryo to study mitosis.

Tags

Microinjection Techniques Studying Mitosis Drosophila Melanogaster Syncytial Embryo Genetics Transgenic Flies Fluorescently Tagged Mitotic Proteins GAL4/UAS System Cell Cycle Duration Imaging Mitosis Rapidly Dividing Nuclei Mitotic Machinery Checkpoint Live Dynamics Function-blocking Antibodies Protein Inhibitors

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