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生于正常化荧光整个心脏成像的光学投影层析成像
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Biology
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JoVE Journal
Biology
Born Normalization for Fluorescence Optical Projection Tomography for Whole Heart Imaging
Please note that all translations are automatically generated.
Click here for the English version.
生于正常化荧光整个心脏成像的光学投影层析成像
DOI:
10.3791/1389-v
•
16:44 min
•
June 02, 2009
•
Claudio Vinegoni
2
,
Daniel Razansky
,
Jose-Luiz Figueiredo
2
,
Lyuba Fexon
2
,
Misha Pivovarov
2
,
Matthias Nahrendorf
2
,
Vasilis Ntziachristos
,
Ralph Weissleder
2
1
Center for Systems Biology
,
Harvard Medical School
,
2
Center for Systems Biology
,
MGH – Massachusetts General Hospital
,
3
Institute for Biological and Medical Imaging
,
Technical University of Munich and Helmholtz Center Munich
Chapters
00:05
Title
00:55
Introduction
01:49
An Optical Projection Tomography Microscope
04:51
Preparing Tissue for Tomographic imaging
09:30
Absorption Reconstructions
11:36
Fluorescent Reconstructions
15:44
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们建议一个光学投影层析成像出生的规范化方法(BnOPT),占成像样品的吸收性能,以获得准确,定量荧光断层重建。我们利用该算法重建的荧光分子探针分布在小动物内脏。
Tags
Born Normalization
Fluorescence Optical Projection Tomography
Whole Heart Imaging
Three-dimensional Imaging Technique
Developmental Biology
Gene Expression Studies
Optical Transparency
Benzyl Alcohol
Benzyl Benzoate
BABB
Murray’s Clear Solution
Clearing Process
Scattering Contribution
Absorption Contribution
Fluorescence Distribution
Image Artifacts
Quantification Errors
Exogenous Contrast Agents
Endogenous Contrast
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