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Obtaining High Quality RNA from Single Cell Populations in Human Postmortem Brain Tissue
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Obtaining High Quality RNA from Single Cell Populations in Human Postmortem Brain Tissue

Obtaining High Quality RNA from Single Cell Populations in Human Postmortem Brain Tissue

DOI:
10.3791/1444-v

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18:17 min

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August 06, 2009

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, ,
1Department of Structural and Molecular Neuroscience,McLean Hospital, 2Department of Psychiatry,Harvard Medical School, 3Department of Psychiatry,Beth Israel Deaconess Medical Center

Chapters

  • 00:05Title
  • 01:06Introduction
  • 01:43Sectioning of Tissue and Staining of Pyramidal Neurons
  • 05:38Single Cell Laser-Capture Microdissection (LCM)
  • 10:48Isolating RNA
  • 13:39Representative Results
  • 17:38Conclusion

Summary

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Automatic Translation

We describe a process using laser-capture microdissection to isolate and extract RNA from a homogeneous cell population, pyramidal neurons, in layer III of the superior temporal gyrus in postmortem human brains. We subsequently linearly amplify (T7-based) mRNA, and hybridize the sample to the Affymetrix human X3P microarray.

Tags

High Quality RNASingle Cell PopulationsGray Matter ReductionSuperior Temporal GyrusSchizophrenia PatientsGene Expression ProfilesPyramidal NeuronsCerebral CortexLaser-capture Microdissection (LCM)Histogene Staining SolutionArcturus XT LCM SystemT7-based Linear AmplificationExperion LabChip (Bio-Rad) GelElectropherogramMRNATranscript LengthMicroarraysHuman X3P GeneChip Probe ArrayAffymetrix

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