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重新用四个转录因子的小鼠胚胎成纤维细胞诱导多能干细胞的产生，强力霉素诱导慢病毒转导系统
JoVE Journal
Biology
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JoVE Journal Biology
Generation of Induced Pluripotent Stem Cells by Reprogramming Mouse Embryonic Fibroblasts with a Four Transcription Factor, Doxycycline Inducible Lentiviral Transduction System
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重新用四个转录因子的小鼠胚胎成纤维细胞诱导多能干细胞的产生，强力霉素诱导慢病毒转导系统

DOI:
10.3791/1447-v

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11:48 min

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November 13, 2009

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, , ,
1Stemgent, 2Whitehead Institute for Biomedical Research,MIT – Massachusetts Institute of Technology

Chapters

  • 00:00Title
  • 00:09Introduction
  • 00:30Viral Transduction of Mouse Embryonic Fibroblasts
  • 01:25Doxycycline Induced Reprogramming
  • 03:18Immunocytochemical Analysis of Transduction Efficiency
  • 05:31Isolating and Expanding Induced Pluripotent Stem Cell Colonies
  • 07:39Immunocytochemical Analysis for Pluripotency
  • 09:14Pluripotency Marker Analysis
  • 10:31Conclusion

Summary

Automatic Translation

Automatic Translation

Stemgent阿霉素诱导小鼠TF慢病毒设置可以重新编程诱导多能干细胞（iPS细胞）的小鼠胚胎成纤维细胞（MEFs）。在这里，我们展示了鼠标重新编程转录因素Oct4的DOX诱导表达的协议，SOX2，KLF4和c - Myc基因产生的iPS殖民地，表达共同的MES多能性标志物。

Tags

Induced Pluripotent Stem CellsReprogrammingTranscription FactorsDoxycycline Inducible Lentiviral Transduction SystemMouse Embryonic FibroblastsPluripotencyRetrovirus-mediated DeliveryOct4Sox2C-MycKlf4Doxycycline Inducible Lentiviral Delivery SystemAdult Mouse Somatic Cell TypesEmbryonic Stem CellsRegenerative MedicineTeratoma FormationDifferentiated CellsEthical DilemmaStemgent DOX Inducible Mouse TF Lentivirus Set

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