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Generation of Induced Pluripotent Stem Cells by Reprogramming Mouse Embryonic Fibroblasts with a Four Transcription Factor, Doxycycline Inducible Lentiviral Transduction System
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Generation of Induced Pluripotent Stem Cells by Reprogramming Mouse Embryonic Fibroblasts with a Four Transcription Factor, Doxycycline Inducible Lentiviral Transduction System

Generation of Induced Pluripotent Stem Cells by Reprogramming Mouse Embryonic Fibroblasts with a Four Transcription Factor, Doxycycline Inducible Lentiviral Transduction System

DOI:
10.3791/1447-v

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11:48 min

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November 13, 2009

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, , ,
1Stemgent, 2Whitehead Institute for Biomedical Research,MIT – Massachusetts Institute of Technology

Chapters

  • 00:00Title
  • 00:09Introduction
  • 00:30Viral Transduction of Mouse Embryonic Fibroblasts
  • 01:25Doxycycline Induced Reprogramming
  • 03:18Immunocytochemical Analysis of Transduction Efficiency
  • 05:31Isolating and Expanding Induced Pluripotent Stem Cell Colonies
  • 07:39Immunocytochemical Analysis for Pluripotency
  • 09:14Pluripotency Marker Analysis
  • 10:31Conclusion

Summary

Automatic Translation

Automatic Translation

The Stemgent Dox Inducible Mouse TF Lentivirus Set can reprogram mouse embryonic fibroblasts (MEFs) to induced pluripotent stem (iPS) cells. Here we demonstrate the protocol for DOX-inducible expression of mouse reprogramming transcription factors Oct4, Sox2, Klf4 and c-Myc to generate iPS colonies that express common mES pluripotency markers.

Tags

Induced Pluripotent Stem CellsReprogrammingTranscription FactorsDoxycycline Inducible Lentiviral Transduction SystemMouse Embryonic FibroblastsPluripotencyRetrovirus-mediated DeliveryOct4Sox2C-MycKlf4Doxycycline Inducible Lentiviral Delivery SystemAdult Mouse Somatic Cell TypesEmbryonic Stem CellsRegenerative MedicineTeratoma FormationDifferentiated CellsEthical DilemmaStemgent DOX Inducible Mouse TF Lentivirus Set

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