A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:00Title
- 00:46Introduction
- 01:15Transfection of 293 Cells To Produce Lentivirus
- 03:24Lentivirus Harvest
- 04:39Lentivirus Concentration via Ultracentrifugation
- 07:24Lentivirus Titration Using Flow Cytometry
- 11:17Conclusion
Tags
Lentivirus ProductionRNA InterferenceGene SilencingShort Interfering RNAs (siRNA)Post-transcriptional StateShort Hairpin RNAsLentivirusesPackaging LentivirusesLentiviral Vector Production SystemsHelper Plasmid ConstructsReplication-capable VirusTransient TransfectionHuman 293 CellsIncubation PeriodVirus TiterReporter ExpressionFlow Cytometer
