Culture of Mouse Neural Stem Cell Precursors

D. Spencer Currle; Jia Sheng Hu; Aaron Kolski-Andreaco; Edwin S. Monuki

doi:10.3791/152

Journal

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Biology

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Cultura de rato precursores das células-tronco neurais

JoVE Journal
Biology

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JoVE Journal Biology

Culture of Mouse Neural Stem Cell Precursors

Cultura de rato precursores das células-tronco neurais

DOI:

10.3791/152-v

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27:44 min

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February 25, 2007

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D. Spencer Currle, Jia Sheng Hu, Aaron Kolski-Andreaco, Edwin S. Monuki

¹Department of Developmental and Cell Biology,University of California, Irvine (UCI), ²Department of Pathology,University of California, Irvine (UCI), ³Department of Physiology and Biophysics,University of California, Irvine (UCI)

Chapters

00:36Preparing plate
05:10Instruments
05:39Dissection of E12.5 mouse
07:55Removal of embryos
13:20Isolation of cortex
18:56Obtaining cell suspension
23:21Plating neural precursors

Summary

Automatic Translation

Este vídeo descreve o método utilizado para o isolamento de neuroprecursors desenvolvimento do córtex de ratos embrionárias. O procedimento para a remoção de embriões do útero, dissecando o tecido cortical, e digerindo o córtex cerebral isolado é mostrado.