Journal
/
/
Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources
DOI:

15:28 min

September 03, 2009

, , , , , ,

Chapters

  • 00:00Title
  • 01:03Introduction
  • 01:39Amplification of Primary DNA LIbrary by PCR
  • 04:41Primer Extension
  • 08:13Extension Product Capture
  • 12:17Capture Product Amplification
  • 12:52Representative Results: PEC Retrieval of Neanderthal Mitochondrial Genomes
  • 14:45Conclusion

Summary

Automatic Translation

We present a method of targeted ancient DNA sequence retrieval, which we used to reconstruct the complete mitochondrial genomes of five Neandertal individuals. Comparison of these sequences with present day humans suggests that Neandertals had a long term low effective population size.

Related Videos

Read Article