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Chapters
- 00:00Title
- 00:46Introduction
- 01:09Aligning the Optics
- 02:51Determining Safe Monitor Powers
- 03:54Determinging the Input Parameters to the Scaler and Pulse Generator
- 06:00Representative Results
- 06:31Conclusion
Bu yazıda, photobleaching sonra çok foton floresan kurtarma kullanarak difüzyon katsayıları ölçmek için prosedür anlatacağız. Biz örnek optik yol boyunca lazer hizalayarak ve uygun deneysel parametrelerin belirlenmesi ile başlayacak, daha sonra devam üreten ve nihayet uydurma floresan kurtarma eğrileri.
Tags
Diffusion CoefficientsTwo-photon Fluorescence Recovery After PhotobleachingMulti-fluorescence Recovery After PhotobleachingMicroscopy TechniqueMacromoleculesIn VitroIn VivoBiological SystemsPhotobleachingLaser FlashFluorescence MonitoringPockels CellLaser ModulatorOptical PathSample PreparationAqueous Fluorescent DyeExperimental ParametersMonitor PowerBleaching PowerBleach DurationBin WidthsFluorescence Recovery TimeRecovery CurvesLabVIEW AutomationEnhanced ThroughputMathematical Model Fitting Algorithm
