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微流控自动化血液循环核细胞富集的裂解协议

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Biology

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JoVE Journal Biology

Automated Microfluidic Blood Lysis Protocol for Enrichment of Circulating Nucleated Cells

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微流控自动化血液循环核细胞富集的裂解协议

DOI:

•

09:53 min

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December 31, 2009

•

William N. White, Palaniappan Sethu

¹Department of Mechanical Engineering,University of Louisville, ²Department of Bioengineering,University of Louisville

Chapters

00:00Title
00:40Introduction
01:13Priming the Microfluidic Device
02:39Preparing the Syringe Pumps
05:01Erythrocyte Lysis
08:32Representative Results/ Outcome
09:20Conclusion

Summary

Automatic Translation

发展循环通过红细胞裂解，以确保高质量的样品无细胞损失隔离外周血有核细胞富集自动化微流体装置。

Tags

Automated Microfluidic Blood Lysis Circulating Nucleated Cells CNCs Enrichment Microfluidic Protocol Device Fabrication Sample Collection Device Setup Blood Lysis Erythrocyte Lysis Herringbone Structures Isotonic Conditions Paraformaldehyde Fixation Flow Cytometry Analysis Disease Pathogenesis Analysis

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