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Chapters
- 00:00Title
- 00:31Introduction
- 02:14Administering Tamoxifen by Oral Gavage
- 04:17Whole Mount Microscopy of E12.5 Embryos
- 05:10Craniotomy and Adult Whole Mount Microscopy
- 08:03Explant Preparation and Micro-Dissection of the Ventral Mesencephalon from E12.5 Embryos
- 09:59Representative Results for Wnt1 Fate-Mapping
- 12:11Conclusion
Fate mappatura genetica inducibile (GIFM) segna e cellule brani con fini di controllo spaziale e temporale<em> In vivo</em> E chiarisce come le cellule da un lignaggio genetico specifico contribuire a sviluppare e tessuti adulti. Dimostrato qui ci sono le tecniche richieste per il destino mappa E12.5 embrioni di topo per l'analisi e epifluorescente espianto.
Tags
Genetic Inducible Fate MappingFate MapsMarking And Tracking Cells In VivoProgenitorsSpecific StructuresCell TypesGenetic Inducible Fate Mapping (GIFM)Gene ExpressionCell FateCell BehaviorsX-CreER LinesBacteriophage ProteinCre RecombinaseCreERTModified Estrogen Receptor Ligand Binding DomainTamoxifenRecombinationLoxP SitesStop CassetteReporter GeneGFPMice BreedingConditional Reporter Allele
