A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:00Title
- 00:47Introduction
- 01:18Assembling Injection Slides and Imaging Slides
- 02:36Injecting DNA Constructs
- 04:43Mounting Embryos for Imaging
- 06:524D Imaging of Cell Behaviors on Olympus FV1000 Confocal with IX81 Microscope
- 08:49Behavior of Individual Labeled Neurons and Neural Crest Cells
- 10:23Conclusion
Tags
Live ImagingCell MotilityActin CytoskeletonIndividual NeuronsNeural Crest CellsZebrafish EmbryosDevelopmentIn VivoOptical ClarityHigh Resolution ImagingCell BehaviorsMigrationAxon OutgrowthGuidanceMechanismsCell LabelingPlasmid DNA InjectionTransient Mosaic Expression PatternProtrusive ActivityMolecular DynamicsTransgenic LinesFine ProtrusionsChanges In Molecular Distribution
