A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:00Title
- 00:46Introduction
- 01:12Antigenic Stimulation
- 02:38Immunofluorescent Staining of Surface Markers for FACS
- 04:25Intracellular Cytokine Staining
- 05:27Representative FACS Data
- 06:29Conclusion
Tags
Multicolor Flow CytometryCellular Immune ResponseRhesus MacaquesHIV-AIDS ModelPathogenesisVaccinesTherapeuticsPhenotypic AnalysisFunctional AnalysisIntracellular Cytokine ProductionCD4+ T CellsCD8+ T CellsMemory SubsetsInterferon Gamma (INF-)Interleukin (IL)-2PMA Plus Ionomycin (PMA+I)Cytokine ProfilesMemory Cells SubsetsAntigen-specific CD4+ T CellsAntigen-specific CD8+ T CellsPBMC SamplesHIV Envelope Peptide Cocktail Vaccine
