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Assaying the Ability of Diffusible Signaling Molecules to Reorient Embryonic Spinal Commissural Axons
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Assaying the Ability of Diffusible Signaling Molecules to Reorient Embryonic Spinal Commissural Axons

Assaying the Ability of Diffusible Signaling Molecules to Reorient Embryonic Spinal Commissural Axons

DOI:
10.3791/1853-v

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09:28 min

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March 08, 2010

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, , ,
1Department of Biological Sciences,University of Southern California, 2Neuroscience Graduate Program,University of Southern California

Chapters

  • 00:00Title
  • 00:28Introduction
  • 01:14Preparation of Aggregates of Transfected COS Cells Using Hanging Drops
  • 02:20Preparation of Dorsal Spinal Cord Explants
  • 03:31Priming Collagen
  • 04:31Dissection of Dorsal Spinal Cord Explants and Positioning with COS Cell Aggregates in Collagen Matrix
  • 06:51Visualization of Commissural Axons By Immunohistochemistry
  • 08:03Representative Images of Explants
  • 08:41Conclusion

Summary

Automatic Translation

Automatic Translation

This assay assesses the ability of a signaling molecule, here Bone Morphogenetic Protein 7 (BMP7), to reorient commissural axons. An explant of embryonic dorsal spinal cord is cultured adjacent to an aggregate of COS cells secreting the candidate growth factors. Reoriented commissural axons growing within the explant are visualized by immunohistochemistry.

Tags

AxonsSignaling MoleculesCommissural AxonsReorientation AssayExtrinsic SignalsIntrinsic SignalsAxon GuidanceBMP ChemorepellentRoof PlateNetrinSonic Hedgehog (Shh)Floor PlateSpinal CordEmbryonic Day (E) 11 RatsCollagen GelsDorsal Spinal ExplantsTag1 GlycoproteinAxonal TrajectoryCOS Cell Aggregate

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