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Journal
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Biology
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Bradford蛋白含量的线性
JoVE Journal
Biology
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JoVE Journal
Biology
Linearization of the Bradford Protein Assay
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Click here for the English version.
Bradford蛋白含量的线性
DOI:
10.3791/1918-v
•
06:35 min
•
April 12, 2010
•
Orna Ernst
,
Tsaffrir Zor
1
Department of Biochemistry
,
Tel Aviv University
Chapters
00:00
Title
00:43
Introduction
01:12
Linearization of the Bradford Protein Calibration Graph
02:55
Measuring Protein Concentration Using the Improved Bradford Protein Assay
04:59
Improved Bradford Protein Assay Representative Results
05:53
Conclusion
Summary
Automatic Translation
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Automatic Translation
受到损害的内在非线性的快速,便捷的Bradford法测定蛋白质的准确性和灵敏度。我们展示一个简单的的线性过程,大大提高了准确性,提高约10倍的检测灵敏度,并显着减少洗涤剂干扰。
Tags
Biology
590 Nm
Coomassie Brilliant Blue Assay
Protein Quantification
Cell Lysates
Cellular Fractions
Recombinant Protein Samples
Normalization Of Biochemical Measurements
Nonlinearity
Sensitivity
Accuracy
Standard Assay Conditions
Absorbance Ratio
450 Nm
Linear Equation
Mass Action
Beer’s Law
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