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Chapters
- 00:20Step 1: cleaning of the sample plate
- 00:46Step 2: preparation of the thin layer substrate solution
- 00:58Step 3: making the thin layer substrate
- 01:52Step 4: preparation of the matrix solution
- 02:05Step 5: test of the thin layer
- 02:50Step 6: sample application
- 03:47Step 7: calibrant application
- 04:20Step 8: washing step
- 05:05Step 9: acquisition of mass spectra
Tags
MALDISample PreparationUltra Thin Layer MethodMatrix-Assisted Laser Desorption Ionization Mass SpectrometryMatrix CrystalsAlpha-cyano-4-hydroxycinnamic AcidSubstrate LayerCrystallizationAdvantagesGreater Tolerance To ImpuritiesBetter ResolutionHigher Spatial UniformityAccurate Mass DeterminationMembrane ProteinsIon ChannelsMetabolite TransportersReceptorsTransmembrane DomainsSoluble ProteinsMolecular MassesHigh-quality Spectra
