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组织测定动物第移植（ACT）的含量非洲爪蟾</em
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JoVE Journal Biology
Tissue Determination Using the Animal Cap Transplant (ACT) Assay in Xenopus laevis
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组织测定动物第移植（ACT）的含量非洲爪蟾</em

DOI:
10.3791/1932-v

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11:00 min

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May 16, 2010

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,
1Department of Ophthalmology,SUNY Upstate Medical University

Chapters

  • 00:00Title
  • 00:59Introduction
  • 01:24Setting Up for the ACT Assay
  • 03:07Preparing for Microinjection
  • 05:18Microinjection
  • 06:30Isolating Animal Caps from Injected Embryos
  • 07:49Transplantation of Caps onto Flank
  • 09:53Animal Cap Transplant Assay Representative Results
  • 10:34Conclusion

Summary

Automatic Translation

Automatic Translation

动物帽高表达的基因产物（S）移植到发展中国家的侧翼<em>非洲爪蟾</em>胚胎，以建立组织是否确定。

Tags

Animal Cap Transplant AssayXenopus LaevisProteinsEmbryonic DevelopmentCell Fate DeterminationNoggin OverexpressionEye FormationComplex PhenotypeEye-specific RoleCell Fate Determination AssayGene ProductsEye Field Transcription Factors (EFTFs)Retinal Cell FateGene Product Identification

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