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薄切片进行免疫组化切片的筹备工作
JoVE Journal
Biology
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JoVE Journal Biology
Thin Sectioning of Slice Preparations for Immunohistochemistry
DOI:

08:12 min

April 28, 2007

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Chapters

  • 00:05Introduction
  • 00:30Mold preparation
  • 04:40Sectioning
  • 05:47Immunoreaction

Summary

Automatic Translation

本方法可重复性的低温恒温器的小,难以管理,组织块,如活组织切片检查和脑切片,切片。我们利用一个简单的铝冻结的阶段,以方便处理组织和一个标准的低温​​恒温器,定期制作5-10微米,400微米厚的脑片连续切片。

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