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Chapters
- 00:06Introduction
- 00:18Tools and reagents
- 01:23Preparation of the cryomold
- 04:45Orienting tissue
- 07:52Flash freezing
- 09:39Using cryostat
- 11:33Preparation and mounting tissue for sectioning
- 19:33Sectioning
- 27:36Conclusion
Demonstrated in this video are the techniques for flash freezing and sectioning embryonic brain tissue from mouse. Useful tips for using the cryostat are given, including troubleshooting methods that can be used while cutting to ensure that the resultant tissues sections are free of cracks and other distortions.
