Journal
/
Biology
/
Cryosectioning Yeast Communities for Examining Fluorescence Patterns
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Cryosectioning Yeast Communities for Examining Fluorescence Patterns

Cryosectioning Yeast Communities for Examining Fluorescence Patterns

DOI:
10.3791/50101-v

•

07:03 min

•

December 26, 2012

•

,
1Division of Basic Sciences,Fred Hutchinson Cancer Research Center

Chapters

  • 00:05Title
  • 00:58Fixing Yeast Communities
  • 03:40Sectioning Frozen Communities for Fluorescence Imaging
  • 05:15Results: Fluorescence Images of Vertical Cross-sections of Yeast Communities
  • 06:44Conclusion

Summary

Automatic Translation

Automatic Translation

We present a protocol for freezing and cryosectioning yeast communities to observe internal patterns of fluorescent cells. The method relies on methanol-fixing and OCT-embedding to preserve the spatial distribution of cells without inactivating fluorescent proteins within a community.

Tags

CryosectioningYeast CommunitiesFluorescence PatternsOptical SectioningConfocal MicroscopyTwo-photon MicroscopySpatial OrganizationSaccharomyces CerevisiaeMethanol FixationOCT CompoundCryostatFluorescent ProteinsGene ExpressionMicrobial Communities

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/50101?article_type=v&slug=cryosectioning-yeast-communities-for-examining-fluorescence-patterns

Flash Freezing and Cryosectioning E12.5 Mouse Brain
check_url/50101?article_type=v&slug=cryosectioning-yeast-communities-for-examining-fluorescence-patterns

Microbial Communities in Nature and Laboratory – Interview
check_url/50101?article_type=v&slug=cryosectioning-yeast-communities-for-examining-fluorescence-patterns

Biology of Microbial Communities – Interview
check_url/50101?article_type=v&slug=cryosectioning-yeast-communities-for-examining-fluorescence-patterns

A Microfluidic Device with Groove Patterns for Studying Cellular Behavior
check_url/50101?article_type=v&slug=cryosectioning-yeast-communities-for-examining-fluorescence-patterns

Time-lapse Imaging of Mitosis After siRNA Transfection
check_url/50101?article_type=v&slug=cryosectioning-yeast-communities-for-examining-fluorescence-patterns

Examining the Conformational Dynamics of Membrane Proteins in situ with Site-directed Fluorescence Labeling
check_url/50101?article_type=v&slug=cryosectioning-yeast-communities-for-examining-fluorescence-patterns

Genomic MRI – a Public Resource for Studying Sequence Patterns within Genomic DNA
check_url/50101?article_type=v&slug=cryosectioning-yeast-communities-for-examining-fluorescence-patterns

A Fluorescence Microscopy Assay for Monitoring Mitophagy in the Yeast Saccharomyces cerevisiae
check_url/50101?article_type=v&slug=cryosectioning-yeast-communities-for-examining-fluorescence-patterns

Quantitative Live Cell Fluorescence-microscopy Analysis of Fission Yeast
check_url/50101?article_type=v&slug=cryosectioning-yeast-communities-for-examining-fluorescence-patterns

Acquiring Fluorescence Time-lapse Movies of Budding Yeast and Analyzing Single-cell Dynamics using GRAFTS

Read Article