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Imágenes in vivo de los cultivos transgénicos parásitos Leishmania en un host en vivo
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1Interdisciplinary Immunology Program,University of Iowa, and the VA Medical Center, 2Department of Biochemistry,University of Iowa, and the VA Medical Center, 3Department of Internal Medicine,University of Iowa, 4Department of Molecular Microbiology,Washington University School of Medicine, 5Division of Dermatology,Harbor-UCLA Medical Center, Hanley-Hardison Research Center, 6Interdisciplinary Immunology Program,Iowa City VA Medical Center, 7Departments of Internal Medicine, Microbiology and Epidemiology,University of Iowa
Chapters
- 00:04Title
- 00:54Introduction
- 01:37Infection of Mice with Leishmania
- 03:54Bioluminescent Imaging
- 06:18Data Analysis
- 07:04Representative Results
- 09:17Conclusion
Un<em> In vivo</em> Sistema de imagen se utiliza para generar mediciones cuantitativas de la infección murina con los protozoos tripanosomátidos<em> Leishmania</em>. Este es un método no invasivo y no letales para la detección de parásitos que expresan luciferasa en muchos tejidos en el curso de las enfermedades crónicas<em> Leishmania</em> Spp. infección.
Tags
In Vivo ImagingTransgenic Leishmania ParasitesLive HostLeishmania SpeciesVisceral LeishmaniasisCutaneous LeishmaniasisMouse ModelsParasite BurdensMicroscopyLimiting Dilution AssayQPCR AmplificationIn Vivo Imaging System (IVIS)Bioluminescent SignalLuciferase ExpressionIntradermal InjectionIntravenous InjectionLongitudinal StudiesInter-animal VariationParasite InoculaMultiplication Of ParasitesHigh SensitivityCutaneous Locations
