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Journal
/
Biology
/
3D Culture of Organoids from Murine Intestinal Crypts and Single Stem Cell
/
Organoid Culture from a Single Intestinal Stem Cell
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Organoid Culture from a Single Intestinal Stem Cell
Organoid Culture from a Single Intestinal Stem Cell
3D Culture of Organoids from Murine Intestinal Crypts and Single Stem Cell
DOI:
10.3791/200008-v
•
02:05 min
•
June 02, 2023
•
Yuta Takase
,
Kazuto Fujishima
3
,
Toshio Takahashi
1
Suntory Foundation for Life Sciences
,
Bioorganic Research Institute
,
2
Institute for Integrated Cell-Material Sciences (KUIAS-iCeMS)
,
Kyoto University
,
3
Faculty of Medicine
,
Osaka Medical and Pharmaceutical University
Tags
Organoid Culture
Intestinal Stem Cell
FEB2-positive
Fluorescence-activated Cell Sorting
ECM
Rock Inhibitor
Crypt-villa Structures
Spheroid Formation
Crypt Protrusion
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