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Journal
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Biochemistry
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Stable Isotope Tracing & LC-MS Analysis to Measure DHODH and SDH Activities
/
13C4-Aspartate and 13C5-Glutamine Stable Isotope Tracing, LC-MS Analysis, and SDH Activity Measurement (Video) | JoVE
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal
Biochemistry
13
C
4
-Aspartate and
13
C
5
-Glutamine Stable Isotope Tracing, LC-MS Analysis, and SDH Activity Measurement
13C4-Aspartate and 13C5-Glutamine Stable Isotope Tracing, LC-MS Analysis, and SDH Activity Measurement (Video) | JoVE
Stable Isotope Tracing & LC-MS Analysis to Measure DHODH and SDH Activities
DOI:
10.3791/200017-v
•
04:55 min
•
May 19, 2023
•
Tenzin Tseyang*
1
,
Jonathan Valeros*
1
,
Paula Vo*
1
,
Jessica B. Spinelli
2
1
Program in Molecular Medicine
,
University of Massachusetts Chan Medical School
,
2
Cancer Center
,
University of Massachusetts Chan Medical School
Tags
13C4-aspartate
13C5-glutamine
Stable Isotope Tracing
LC-MS Analysis
SDH Activity Measurement
143B Human Osteosarcoma Cells
Cell Culture
Metabolite Extraction
Vacuum Concentration
Liquid Chromatography
Mass Spectrometry
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