JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biochemistry
/
High-Throughput Quantification of the Synthesis and Distribution of mtDNA
/
用于线粒体DNA合成的siRNA混合物的制备
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biochemistry
Preparation of siRNA Mixture for Mitochondrial DNA Synthesis
Please note that all translations are automatically generated.
Click here for the English version.
用于线粒体DNA合成的siRNA混合物的制备
High-Throughput Quantification of the Synthesis and Distribution of mtDNA
DOI:
10.3791/200039-v
•
00:45 min
•
May 05, 2023
•
Lukasz S. Borowski
2
,
Karolina Kasztelan
3
,
Jolanta Czerwinska-Kostrzewska
2
,
Roman J. Szczesny
1
Faculty of Biology, Institute of Genetics and Biotechnology
,
University of Warsaw
,
2
Institute of Biochemistry and Biophysics
,
Polish Academy of Sciences
,
3
International Institute of Molecular and Cell Biology in Warsaw
Tags
SiRNA
Mitochondrial DNA
Small Interfering RNA
RNAiMAX
Transfection
Microplate
Cell Culture
Reagent Dispenser
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
check_url/200039?article_type=v
基于图像的高通量线粒体DNA合成和分布定量
check_url/200039?article_type=v
用于线粒体DNA合成的siRNA混合物的制备
check_url/200039?article_type=v
细胞转染和5-溴-2'-脱氧尿苷(BrdU)在HeLa细胞中的掺入
check_url/200039?article_type=v
HeLa细胞中线粒体DNA的5-溴-2'-脱氧尿苷(BrdU)标记
check_url/200039?article_type=v
HeLa细胞固定与Hoechst 33342和阻断
check_url/200039?article_type=v
向转染的HeLa细胞中添加一抗和二抗以监测mtDNA分布
check_url/200039?article_type=v
定量图像分析,用于测量转染HeLa细胞中的mtDNA和基因表达水平
Read Article