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Journal
/
Biochemistry
/
High-Throughput Quantification of the Synthesis and Distribution of mtDNA
/
Kvantitativ billedanalyse til måling af mtDNA- og genekspressionsniveauerne i de transfekterede HeLa-celler
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Quantitative Image Analysis to Measure the mtDNA and Gene Expression Levels in the Transfected HeLa Cells
Kvantitativ billedanalyse til måling af mtDNA- og genekspressionsniveauerne i de transfekterede HeLa-celler
High-Throughput Quantification of the Synthesis and Distribution of mtDNA
DOI:
10.3791/200044-v
•
03:02 min
•
May 05, 2023
•
Lukasz S. Borowski
2
,
Karolina Kasztelan
3
,
Jolanta Czerwinska-Kostrzewska
2
,
Roman J. Szczesny
1
Faculty of Biology, Institute of Genetics and Biotechnology
,
University of Warsaw
,
2
Institute of Biochemistry and Biophysics
,
Polish Academy of Sciences
,
3
International Institute of Molecular and Cell Biology in Warsaw
Tags
Quantitative Image Analysis
MtDNA Levels
Gene Expression
Transfected HeLa Cells
Fluorescence Imaging
Background Correction
Image Segmentation
BrdU
Mitochondrial DNA
Quantitative Real-time PCR
Dideoxycytidine
DDC
TFAM
TWINKLE Helicase
POLG
Mitochondrial DNA Polymerase Gamma
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